Study of Some Parameters that Play Role in Pathogenesis of Celiac Disease.

Abstract

Background:Coeliac disease defines as autoimmune disease induced by the effect of wheat proteins which represented by gluten and related proteins from other grains (barley or rye).when exposure to gluten in susceptible individuals, induce the activation of Th1- lymphocytes and up-regulation of interferon-gamma in genetically predisposed individuals, which lead to inflammation in the intestinal mucosa of celiac disease patients. However, a certain level of ROS under physiological conditions in addition to abundant of antioxidant capacity, the cell can tolerate tis oxidative conditions , which is critical for intestinal homeostasis, but when the excessive ROS generation or decreased reduction reactions  this lead to increased oxidant load , as well as reduce the antioxidant activity lead to enhance membrane permeability, in addition to various effect such as inflammatory response and result in lipid and protein modifications, as well as,DNA damage, carcinogenesis, and apoptosis,. Aim of study: investigation some of markers to confirm the celiac disease stimulated factors.Patients and Methods: Study included 20 case CD patients with 12 healthy controls, Serum patients and controls examination in Al-Zahraa hospital, Dep. Immunology.Results: Serum IL-12 levels in patients (-7.20) and controls (-11.23) with highly significant differences (0.034)which mean that IL-12 activated due to ROS produce, MMP-3 in patients (6.27) and controls( 11.72)with sig.0.001,compared with the levels of TIMP-3 which few increased to inhibit the MMP-3 stimulated (11.20) and in controls was (25.33)with sig.o.ooo1. due to the activate of IL-12 in patients serum ,this lead to stimulate production of IFN-gamma which was ( 19.30) in patients , but (11.83) in controls with sig.0.029. Total oxidative status which used to estimate the overall oxidation state of the body was (4.53) in patients, (8.20) in control with sig.0.009, compared with the activity of antioxidant parameter SOD which levels in patients was (12.63) and (21.33) with sig. 0.008.