An analytical intervention of Plectranthus Amboinicus extracted from cow ark attenuating liver enzymes activities and Alpha fetoprotein level in CCL4 induced Hepatocellular Carcinoma

Abstract

Cow ark, (Cow urine distillate), is an indispensible therapeutic agent in traditional Indian medicine and Ayurveda. Incidence of several diseases, including Hepatocellular carcinoma (HCC) is increasing gradually to a new peak. The main objectives of this investigation were to detect and evaluate protective ability of Plectranthus Amboinicus  extracted from cow ark on enzymatic functions of liver and to explore attenuating capability of cow ark on HCC biomarker. The analyses include, effects of Plectranthus Amboinicus  with cow ark (BCA), in serum level of AST,ALT,ALP, Total bilirubin, and alpha fetoprotein (AFP) fluctuations in carbon tetrachloride( CCL4), induced HCC rats. Toxicity was stimulated by CCL4 with olive oil as vehicle in the ratio of (1:1 v/v);( olive oil 5mL/kg i.p). Sylimarin was used as a reference (standard) for intoxicated animal groups. Moreover, observation of histological studies favours the biochemical changes observed. Results showed BCA is highly potential in depleting the level of liver enzymes and AFP biomarker, based on dose regimen. These might have been due to the action of enriched phenolic content of BCA, to get rid of free radicals. Study concluded that BCA is a universal medicine and modulating biochemical changes in hepatotoxicity of liver cells induced HCC rat model.