Formulation and Evaluation of Terbulus terrestris Topical gel for Wound healing activity

Abstract

The aim of the present study is formulation and evaluation of Topical gel Terbulus terrestris (T. terrestris) extract and to evaluate the gel for its physical properties, in-vitro permeation, and wound healing properties in rats. Materials and Methods: Topical gels were prepared using two polymers namely Carbopol and Hydroxypropyl Methyl Cellulose (HPMC) (1-1.5%). The gels were evaluated for their viscosity, spreadability, content uniformity. In-vitro diffusion study was conducted for topical gels using Franz diffusion cell for the biomarker compound β-sitosterol. Results and Discussion:  It was observed that with an increase in the polymer percentage, there was a proportional increase in the viscosity and reduced spreadability. Similarly, the rate of diffusion of the drug from the gels was also reduced with an increase in the polymer concentration. All the prepared formulations demonstrated content uniformity and moderate to good spreadability. Treatment with Topical gel decreased wound contraction time.  The fall of eschar left no raw wound behind (0.373±0.665). Percentage wound contraction  (96.22) in 14-15 days, the results of the wound healing activity were significantly better than the control group. Conclusions: It can be concluded that T. terrestris loaded Topical gels is a viable option for the treatment of wounds.