The Effect of Repaglinie and Metformine on Ovarian Function of Alloxan Induced Diabetes Female Virgin Rats

Abstract

There is association between diabetes mellitus  and abnormal ovarian function. There are many oral hypoglycemic agents that are used to treat diabetes as repalinide and metformin . To estimate the effeciancy of repalinide and metformin in improvement ovarian function virgin female ratswere used in this study.The female divided into two groups the first group were used for the induction of diabetes mellitus typeІІ using alloxan monohydrate. The rats with continual blood glucose level above 200 mg/dl for seven day were considered to be hyperglycemic and were selected for treatment,the second group of female rat were given normal saline intraperitonialy, both two female groups were divided into three subgroups that included. Normal control, Non-diabetic rats treated with repaglinide 4mg/kg/, Non-diabetic rats treated with metformin 1000 mg/kg/day, Diabetic control (DC) ,Diabetic rats with repaglinid 4mg/kg/day and Diabetic rats with metformin 1000 mg/kg/day. After 6 weeks of treatment rats were sacrificed , ovaries were collected and then weighted.Subsequently, histological sections of overies were prepared to investigate the mean total number of follicles and corpus lutea in control and treatment groups to evaluate the effect of treatment on ovarian function. The results revealed significant increase (P≤0.05) of ovarian weight in diabetes with repaglinide group and significant increase (p≤0.05) in secondary follicles number and corpus luteum number in normal with metformin group as compared to normal control group