Pectinases Synthesis by Ralstonia solancearum is influenced by various nutritional and environmental factors

Abstract

Ralstonia solancearum was isolated from four host plants viz. Turmeric, Banana,
Tomato and Brinjal. From these samples total twenty strains were isolated and screened
for further study. Out of twenty strains, one strain is recorded as more effective for the
pectinases (PG, PmG, PGL, PnL) production. This strain was further used for the study of
various factors like time of incubation, temperature, pH, sources of carbon and nitrogen
affecting pectinases production. The optimum time, temperature and pH for pectinases
production was 48hrs, 30 °C and 7 respectively. In the experiment of effect of various
sources of carbon (pectin, sucrose, glucose, maltose, starch) and sources of nitrogen
(yeast extract, ammonium nitrate, ammonium sulphate, potassium nitrate, peptone) it was
found that pectin was most suitable carbon source and yeast extract was most suitable
sources of nitrogen for optimum production of pectinases.