Effect of adding two levels of Resveratrol and Oleuropein to the diet on some biochemical traits of blood and gene expression for heat shock protein (HSP70) in the liver for broiler chickens reared under heat stress conditions

Abstract

The experiment was conducted in the poultry field belonging to the Animal Production Department, the college of Agriculture at Al-Qasim Green University for a period from 5/9/2020 until 6/20/2020. used in the experiment 225 broiler Chicks from the Ross-308 strain at the age of one day. He gave her fodder and water freely adlibitum, Chicks were distributed randomly to 5 treatments at an average of 3 replicates for each treatment, and for each replicate 15 birds, the birds were exposed to a temperature of 36 ± 1 ° C and the humidity 50-60% Chicks were distributed randomly into 5 treatments at a rate of 3 replicates per treatment and each replicate had 15 birds. and the experiment treatments were divided into 5 treatments (T1). A control group fed on a basic diet without adding any substance, (T2 and T3) were fed on a basic diet with the addition of Res at a concentration of 500 and 1000 mg. / Kg of diet, respectively, (T4 and T5) were fed on a basic diet with the addition of Ole at a concentration of 500 and 1000 mg / kg diet respectively. The results of this experiment showed that the adding of Res and Ole led to a significant decrease (p <0.01) in the percentage of H / L and in the concentration of glucose and LDL. Also, a significant decrease (p<0.01) in the concentration of uric acid, ALT and AST enzyme, and a significant increase (P <0.01) in the concentration of total protein, albumin and globulin, and an increase in the concentration of GSH-px enzyme in adding treatments compared with the control treatment T1.Also, a significant decrease (p<0.01) of gene expression HSP70 in the liver for the Res and Ole treatments compared with the control treatment T1, and the T3 treatment recorded the best results.