PCR Detection of Biofilm Gene of Klebsiellaoxytoca isolated from Sinusitis Infections

Abstract

The study aimed to detected  the predominance of biofilm formation gene in Klebsiellaoxytoca isolated from sinusitis infection,  therfor , 156 of sinusitis  swabs were collected from nasal infection,  and cultured on suitable media (Manitol ,MacConkey and Chocolat agar), the  result appear  122 of specimens were  gave bacterial  growth ,primary identification were depend on morphological and culture characters , biochemical test and finally identification with vitek-2 system . The bacterial growth  were represnted by  52(42.6%) of isolates  were gram positive   and  70 (57.4%) were gram negative  bacteria , from gram negative  18(14.7%) of isolates were identified as Klebsiellaoxytoca. genetically PCR technique were used to invistigated thepredominance of study mrkA, fimA , matB, pilQ, pilL,genes encoding biofilm formation in Klebsiellaoxytoca isolates , the result indecated that (50%) of isolates mrkA genes , (66%) from isolates  gave positive result tofimA gene and matB gene were found  in (94%)  of bacterial isolates while  pilL genes were found  in(72%)  and  pilQ genes were  detected in (89%) of  isolates  .