Association of Tumor Necrotic Factor Alpha Gene Single Nucleotide Polymorphism in Rubella Vaccine Response

Abstract

Background:Rubella virus is a mild, acute, contagious disease. Rubella usuallyoccurs in young adults and in children, and mainly transmitted by respiratory route. Pregnant women infected with rubella can transmit infection to their offspring. Congenital rubella cause serious infection include miscarriage,  stillbirth, asymptomatic infection in the newborn and /or Congenital Rubella Syndrome (CRS).

Objective: To detected level of Immune response to Rubella virus vaccine among vaccinated children and to investigate is the single nucleotidepolymorphisms(SNP) inTumor Necrotic Factor Alpha (TNF-α gene)are associated with variations in response to rubella.

Methods: This is a cross-sectional study, a total of 180 hospitalized children age between (6-8) years were included, who are previously vaccinated with two doses ofmeasles-mumps-rubella vaccine) MMR(vaccine. All samples were collected from the unit of blood collection in the Central Teaching Hospital of pediatric in Baghdad/Al-Iskan during the period from October 2019 to January 2020.Level of Immune response of IgG antibody detected by  enzyme linked immunosorbent assay (ELISA), SNP in TNF-αgene detection by allele-specific polymerase chain reaction(PCR).

Result:The number of children who had low immune response was 33,moderate immune response was 103 and the children with high immune response was 44 with percentages 18.34,57.22% and 24.44% respectively. Female was  slightly high percentage with low immune response with  19% than male with17.8% ,while male has high percentage of high immune response with 25.7% than female  with 22.8%  with no significant difference.The percentage of low immune response is more in age 6 and 8 years than 7 years with percentage of 18.8% and 18.7% respectively.

Conclusions:MMR vaccine is important especially in female to avoid congenital infection Age may effect on immune response of MMR vaccine.There is an association between SNP in TNF-α gene and Rubella vaccine immune response.