Association between Interleukin-18 (-607 C→A) Polymorphisms and Risk of Chronic Kidney Disease

Abstract

Background: We designed this study to determine the  association between gene polymorphism of IL-18 (-607 C→A) with the development of chronic kidney disease using ARMS-PCR.The present study detected IL-18 -607 C→Agenotype frequency distribution in CKD patients and control.

Methods: A total number of 113 clinical samples were collected from patients who suffer from chronic kidney disease (CKD) .The complete genomic DNA of 113 patients with CKD and 100 of control was extracted employing a traditional kit of the entire human genomic DNA extraction kit.

Results : The genotypes relative frequency in CKD patients were as  follows. CC (1.76%), CA (98.2%) and AA (0%). C allele has a close frequency in CKD patients with (50.4%) to A allele (49.5%). There was no significant difference (P value = 0.49) among CKD patients and control group. Data of the current study in chronic kidney disease patients showed that CA heterozygote genotype was the most frequent, followed by CC homozygote while there is no AA allele in this study. However, There was no significant differences in the IL-18 allele frequency distribution between control and chronic kidney disease patients in which (P value = 0.49).Conclusions : This study indicate that IL-18 -607 C→A allele do not consider a risk factor for effection on the level of  IL-18 in patients with chronic kidney disease.