Molecular Study for Propionibacterium Acnes Isolated from Acne Volcgaris

Abstract

    In this current study, 120 samples were taken from patients with acne of different ages and from both sexes, who were referred to the outpatient clinics in Al-Muthanna Governorate during the period from September to January 2021. It was cultivated on the transplantation environment, to identify the phenotypic and external characteristics, and to conduct chemical tests, Then it was diagnosed by 16srRNA and after conducting chemical and molecular tests, 49 of the 120 isolates were confirmed as Propionibacterium acnes 15 samples were selected from the positive samples for testing of 16srRNA and diagnosed as Propionibacterium acnes, which was tested for the gene responsible for the lipase enzyme (gehA gene), as well as for the gene responsible for the halurundis enzyme (HYL-IB / II gene), as the most important virulence factors in Propionibacterium acnes because the two enzymes played an important role in bacterial pathogenesis, and the result was 100%.