Effect of local injection of platelet-rich plasma on level of IL-1β and TNF-α in gingival crevicular fluid during orthodontic tooth movement in dogs

Abstract

Objectives: This study sought to assess the effect of local injection of platelet-rich plasma (PRP) on level of interleukin 1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF) during orthodontic tooth movement in dogs.

Materials and Methods: Six dogs, aged 10 to 12  months, were used in this study. one quadrant of the maxilla in six male mixed breed dogs was randomly selected as the test group and the contralateral quadrant served as the control group. After bilateral extraction of maxillary first premolars, a nickel-titanium closed coil spring was placed between the second premolar and canine teeth to apply 200 g force for space closure. A mixture of PRP and thrombin-calcium chloride was injected into the periodontal ligament of the second premolar in the test quadrant at 0, 21 and 42 days. A mixture of thrombin-calcium chloride was injected into the periodontal ligament of the second premolar in the control quadrant as placebo at the same time points. The levels of IL-1β and TNF-α in GCF was measured using ELISA. Data were analyzed using repeated measures ANOVA, Bonferroni test, and independent t-test.

Results: The levels of IL-1β and TNF-α in the GCF of the test group were significantly higher than the corresponding values in the control group at all time points (P<0.001). A significant increase in GCF levels of IL-1β and TNF-α was also noted most all the time (P<0.05).

Conclusion: Local injection of PRP can significantly increase the level of IL-1β and TNF-α in GCF of teeth under orthodontic force application.