Anti-proliferative effect of Ceriopsdecandra derived fraction against Human gastric cancer on AGS cell line

Abstract

Mangrove plant species C,decandra have wide range of pharmacologic actions. Such as anti microbial, Antioxidant, anti-inflammatory and anti oralcancer  activity. The present study aimed to evaluate the antiproliferative and biochemical properties of mangrove derived on human gastric cancer on AGS cancer.Theanti proliferative effect of C.decandra isolated bioactive compound berberine was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation method along with assessment of changes in the enzymatic and non enzymatic antioxidant status. We observed intracellular reactive oxygen species(ROS) level by DCFH-DA method, mitochondrial membrane potential alteration by rhodamine 123 staining and apoptotic morphological changes by acridine orang/ethidium bromine dual staining method and Hoest staining assay and comet assay. Mangrove derived berberine enhances lipid peroxidative markers such thiobarbituric acid reactive substance conjugated diene, and lipid hydroperoxide in AGS cell lines. Mangrove derived fraction enhances the ROS levels, which is evidenced by the increased 2-7 diacetyl dichlorofluorescein fluorescence. Further, C.decandratreatment altered the mitochondrial membrane potential in AGS cells. Additionally, we observed increased apoptotic morphological changes in berberine treated group.Mangrove fraction exhibit potent antiproliferative effect on AGS cell line and upon further will going to check invivo experimental animal studies.