Antioxidant activity in aqueous and methanol extract of combinations of Psidiumguajava, Foeniculumvulgare and Nigella sativa

Abstract

Background: Natural antioxidants are widely distributed in food and medicinal plants. These natural antioxidants, especially polyphenols and carotenoids, exhibit a wide range of biological effects, including anti-inflammatory, anti-aging, anti-atherosclerosis and anticancer.

Objectives: To study the antioxidantactivity in aqueous and methanol extract of combinations of Psidiumguajava, Foeniculumvulgare and Nigella sativa.

Methods: The antioxidant activity was done as per the standard methods in the aqueous and methanolic extract of Psidiumguajava, Foeniculumvulgare and Nigella sativaby usingDPPH free radical scavenging activity, FRAP assay, Superoxide scavenging assay, phosphomolybdenum complex.

Results&Discussion:Our results show high DPPH scavenging activity.Aqueous extract of combination of Psidiumguajava, Foeniculumvulgareand Nigella sativa showed the maximum level of inhibition in FRAP assay and it was found to be 97% likewise aqueous extracts of combination of Psidiumguajava, Foeniculumvulgareand Nigella sativa also showed the maximum level of inhibition and was found to be 88 % respectively. Aqueous and methanol extract of combination of Psidiumguajava, Foeniculumvulgareand Nigella sativa showed a concentration-dependent increase in the inhibition of superoxide generation and the highest scavenging activity for O2 •-, was observed at a concentration of 120μg/ml. Total antioxidant capacity was analysed by using Phosphomolybdenum complex.  In our study aqueous and methanol extract of combination of Psidiumguajava, Foeniculumvulgareand Nigella sativa showed a maximum level of antioxidant capacity and it was found to be more than 70% This could due to the high contents of total phenolics and flavonoids in these extracts.

Conclusion: In the present study, we have investigated the antioxidant potential of Psidiumguajava, Foeniculumvulgareand Nigella sativa. Therefore, it would seem likely that both solvents were able to extract those compounds which are responsible for the antioxidant activity of the plant. Hence, our study concludes that Psidiumguajava, Foeniculumvulgareand Nigella sativa can be considered as potential antioxidant natural drugs for the treatment of various diseases.