Protein extractionfrom muscle tissue of Scomberomorouscommerson(Spanish Mackerel) using 2D Electrophoresis and SDS PAGE

Abstract

2-D electrophoresis and SDS-PAGE are being pioneer in protein identification and evaluation, proteomics profiling of this species via 2DE and SDS- PAGE could help in identify various protein components along with its metabolic function.Spanish Mackerel (Scomberomoruscommerson) being economically important species and consumed worldwide, proper nutritional profile is still in research. Proper protein profiling of this species could lead to discovery of many nutritional facts. Present study focused on protein extraction of this species using 2DE and SDS-PAGE and obtained approximate molecular weight lying between 37 and 145 kDa in 13cm IPG strips.