Evaluation of Effect of Neem Oil as a Deliver Vehicle on Antimicrobial Property of Triple Antibiotics Paste: An In Vitro Study

Abstract

Introduction: Probably microbial plaque is the main etiology for periodontal tissue inflammation. Various chemical agents have been evaluated over the years with respect to their antimicrobial effects in the oral cavity. However, all are associated with side effects that prohibit regular long-term use. Hence, in the present study weevaluated the effect of neem oil as a deliver vehicle on antimicrobial property of triple antibiotics paste.

Materials and Methods: Culture was prepared using brain heart infusion broth reagent. Recently extracted intact single rooted human single rooted single canal teeth were selected and sterilized. Three types of microorganisms isolated were used. A mixed suspension of equal volumes of the three organisms was prepared in sterile containers. Neem oil was kept in the agar plate with culture and the diameter of inhibition zones was calculated.

Results: After 48 h, inhibition zones were formed on the agar plates impregnated with neem oil. The inhibition zone signifies the reduction of microorganisms on the agar plate. These inhibition zones on the agar plates looked circular in shape and the zones were measured with the help of a millimeter scale.

Conclusion: This in vitro study concludes that the commonly used natural herbs among the rural people, such as neem, have beneficial antimicrobial activity. Hence, further studies are required in formulating the anti-plaque agents based upon herbs like A. indica.