Phylogenetic Analysis of Proteus SPP from Cave-Dwelling Bats That are Risk to Human in Iraq

Abstract

The present study aimed to describe the genetic relationships of zoonotic characterization of proteus isolated from intestine of Bat and close relation with proteus that caused disease to the human. The study includes (40) Bat insect ivory species Myotisemarginated. The isolation and identification of Proteus Spp were done by using enrichment culture method, and then confirmed by PCR technique based on 16S ribosomal RNA gene which designed in this study using NCBI-GeneBank (CP034105.1) and DNA sequencing was done on some positive isolates. The results show that proteusspp was isolated from intestine of Bat 20(50%) by culture method. The PCR technique was show highly sensitive and specific confirmative detection of Proteus sppisolates result occur about 10(25%) at Clarify DNA sequencing of a partial sequence of 16S ribosomal RNA gene was shown homology sequence identity highly with NCBI-Blast Proteus isolates. The phylogenetic analysis was show clear genetic similarity at (0.5 genetic change) between proteusmiribilus and proteus vulgaris isolates. The gene sequence deposited into NCBI-GenBank accession numbers (MH119074.1, MH119075.1). In conclusion, the study presents focus on main pathogen that infects Bat in Iraq of genetic relationship among Proteus sppisolates Bat. Therefore, it is essential to define the role of Bat an important source for the distribution of pathogen related to public health