Screening of Diabetes Mellitus Induced by Alloxan in Wister Rat Using FTIR - ATR Spectral , Biochemical and Histological Studies Approach

Main Article Content

Saira Khatheeja, Safiullah A

Abstract

Screening of diseases in the medicine plays vital role in identification , treatment and management of diseases. Though we have   numerous methods  for screening Diabetes Mellitus  and each methods has its own specificities concern with principles and technologies adopted. In this current research work,  FTIR ATR techniques was adopted to screen the Diabetes Mellitus  along with biochemical and histological analysis as comparison and supportive.  Experiments were conducted on  Diabetes Mellitus induced   Wister rat with alloxan for 30 days followed by termination with analyzing Biochemical, FTIR-ATR spectral and histological studies . The results obtained were more significant for glucose, Triglycerides cholesterol , HDL cholesterol were  at p<0.0001 level and incase of TSH was  significant at  p< 0.05 level  , the other parameters studied  were statistically not significant.


The FTIR-ATR study shows that    successful spectral differentiation for control and diabetic sample obtained  between  3283 – 532  cm-1 . The Significant elevation in the absorption peak ratio was documented in  (Protein )asym and Sym   to   TGL / HDL- cholesterol ester in diabetic induction on rat by alloxan. The  slight variation peak absorption ratio for  Amide II  to Amide III and Nucleic acid-PO2 as well as Glucose Stretching to  S-S stretch in cystic acid  were noticed.  FT-IR  spectral results  in different  tissues  of Alloxan induced  diabetes  shows the intensity of the band at 1040 cm−1, which is due to oligosaccharides,


Histological  studies shows thickening of parietal layer of Bowman’s capsule of kidney, inflammation  in thickened alveolar septae  and blood vessels  of lungs  but in case of liver muscle and heart no appreciable  changes recorded .The importance of FTIRATR spectroscopic technique in screening of Diabetes Mellitus is discussed and recommended as diagnostic tool in medical field.

Article Details

How to Cite
Saira Khatheeja, Safiullah A. (2021). Screening of Diabetes Mellitus Induced by Alloxan in Wister Rat Using FTIR - ATR Spectral , Biochemical and Histological Studies Approach. Annals of the Romanian Society for Cell Biology, 6786–6802. Retrieved from https://www.annalsofrscb.ro/index.php/journal/article/view/848
Section
Articles