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Pseudomonas aeruginosa is an opportunistic bacterium that lives in all wet and dry environments and animal tissues. It is also one of the main pathogens, especially in patients with the urinary tract, burns, cystic fibrosis, and bacteremia. P. aeruginosa produces several extracellular pigments that are important virulence factors associated with its pathology and virulence, among them is pyocyanin pigment. This study was designed to the green synthesis of gold nanoparticles and study their effect on the production of pyocyanin pigment in local Pseudomonas aeruginosa isolates. All isolates were identified as P. aeruginosa according to morphological, cultural, biochemical characteristics, VITEK-2 and 16S rRNA. AuNPs were prepared using black tea leaf extract and characterized by UV-vis, XRD, SEM, FT-IR analysis. The results showed that the pyocyanin pigment production increased under the influence of gold nanoparticle with concentrations 20, 40, 80, 160, 320, and 100000 µg / ml.