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The main objective of this study was to detectionthe presence of OTA in wheat and maize grains in Babylon Governorate in Iraq.. Wheat (Triticumaestivum) and maize (Zea may) grains were used todetect OTA concentrations. The presence of OTA in grains was determined by TLC andHPLC. The results of TLC indicated that 5 of 6 strains of A.niger was produced of OTA and 4of 4 strains ofA.ochraceus produced of OTA. The results of HPLC indicated that the concentration of OTA extract from A.ochraceus that isolated from maize grains recorded the highest concentration (98.3 , 124.9) ppb respectively, while the concentration of OTA extract from A.niger that isolated from wheat grains recorded the highestconcentration(8.14, 3.50) ppbrespectively, than that isolate from maize grains.