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The study was aimed to determine the effect of oral dosage of AgNPs or Bifidobacterium bifidum on some biological and immunological parameters of male laboratory rats after fed with 2.5 mg of aflatoxin/ kg feed for 21 days. The results showed that there was a significant increased (p<0.05) in the relative weights of the liver, kidneys and spleen in the fed groups compared with the group of rats given aflatoxin alone. Also, the concentrations of liver enzymes AST and ALT and immunoglobulins of IgA, IgM, IgG and IgE were significantly increased compared with the Rats group that treatment with fed with aflatoxins. The oral dosage of AgNPs or Bifidobacterium bifidum were return the biological and immunological parameters of male laboratory rats significantly to the same values in male rats in control group.