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Aim: The study aims to investigate the efficacy of Sonic activation and Blue flex canal brush for smear layer removal using Scanning Electron Microscopy (SEM).
Materials and method: Sixty mandibular premolar were selected for the study. All the specimen were randomly distributed into the following 3 group: Group I (control): Conventional irrigation by Syringe. Group II: Sonic activation. Group III : Blue flex canal brush. In all the groups mechanical instrumentation were done with protaper gold rotary file. After instrumentation the teeth were grooved vertically on the buccal and lingual surfaces using a water-cooled diamond bur. The teeth were split along the long axis in a buccolingual direction using a surgical chisel. These specimens were mounted on a metallic stub and sputter coated with 20nm layer of gold and subjected to SEM evaluation.
Results: Statistically significant difference was found between the three experimental groups. Amid all the groups maximum smear layer was removed with Blue flex canal brush group and when used in combination with conventional irrigation.
Conclusion: All the groups similarly removed smear layer, but maximum was seen with Blue Flex Canal brush and least with conventional irrigation among the experimental groups.