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Four hundred midstream urine sample (MSU) were collected from students apparently healthy in ten primary schools in Al-Muthannaa city from the beginning of October 2019 to the end of February 2020. The collected urine were culture on different media (MacConkey agar, Eosin Methylene Blue), and tested biochemically in order to find out the profile of E.coli. polymerase chain reaction technique has been used to detect some of virulence genes Flu, and CsgA which encoding by E.coli isolates. The results demonstrated that out of total 40 isolates (20 urine + 20 stool) there were 25 isolate (62.5%) was positive for Flu gene, The prevalence of Flu gene in commensal was significantly lower (50%) than that in recurrent higher UPEC (75%). 31 isolate (77.5 %) were positive for CsgA gene, The result found a high prevalence (80%) of CsgA in UPEC isolates, while CsgA gene was detected in (75%) of the intestinal commensal isolates . In this study, describe the profile of E.coli bacteria isolated from healthy Iraqi children with no symptoms, for UTI or diarrhea by the identification of virulence genes.