Evaluation of Lytic Bacteriophages for Control of Multidrug-Resistant Salmonella Typhi

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Mohammed N. Hussein, Wafaa A. Jahell, Thifaf J.M. Al-Shekake,Ziad M.F. Al-khozai


The introduction of antibiotic-resistant bacteria can cause severe clinical and public health concerns. This research serves as an indication of using bacteriophages to control multidrug-resistant bacterial Salmonella Typhi as an alternative agent. Two anti-Salmonella lytic bacteriophages were isolated from sewage water, primarily called Phage 1 and Phage 2. The large host spectrum of Phage 1 was lytic against all Salmonella isolates included in the analysis. In comparison, phage 2 has a small host range since only 25 isolates could be inhibited, and there are no phages of Discouraged bacteria other than Salmonella spp. Adsorption rate (Phage 1) was (2.2 x 10-10) ml one minute, Adsorption rate (phage 2) was (1.8 x 10-10) ml one minute. Phage 1 eclipse and latent cycles were 5, 11 minutes and 6.5, 13 minutes respectively for phage 2; Phage 1 and 2 burst sizes were 78 ± 15 pfu\cell and 61 ± 10 pfu\cell, respectively. The complete Phage 1 particle lysis period was four hours, while the complete Phage 2 particle lysis time was 5 hours. Phage passage findings showed a substantial increase in PFU, which reached its maximum elevation in Phage 1 at six passages and in Phage 2 at the fourth passage. Phage 1 and Phage 2 fitness for six generations were 23 ± 5 pfu\cell and 20 ± 2 pfu\cell, respectively. The particles of Phage 1 and 2 were stable at a broad ph range (6-10) at (30-50) °C, with an optimum tem. of 37 °C for the two phages

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Thifaf J.M. Al-Shekake,Ziad M.F. Al-khozai, M. N. H. W. A. J. . (2021). Evaluation of Lytic Bacteriophages for Control of Multidrug-Resistant Salmonella Typhi. Annals of the Romanian Society for Cell Biology, 25(6), 11359–11372. Retrieved from https://www.annalsofrscb.ro/index.php/journal/article/view/7643