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The current research includes conducting a biological study in the study stations, which diagnosed some indicators of bacterial contamination of water and included (the total number of plate count total bacteria, the total number of total coliform bacteria and fecal coliform bacteria) Faecal, (Coliform, and the study began in October 2020 until March 2021). Six stations were chosen and distributed as follows: the first station on the course of the Zab River, and the other stations on the Hawija water purification project (the drawing area, collection basins, sedimentation basins and purified water tap water) The water samples were taken with the aim of analyzing them in the laboratory and determining some of their biological characteristics, in addition to that some readings were taken in real time The results of the current study showed that the project water in general is contaminated with bacteria (EPA, 2002) The total numbers of bacteria were not in conformity with the standard specifications for drinking water. Iraqi (Standard No. 417 for drinking water for the year 2001) and amounting to 10 cells/ml.