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The study detects microbial contamination of computer keyboards in Pharmacy College /Baghdad University and several internet centers in Baghdad/Resafa side. Samples were collected from 20 computers in Pharmacy College and 80 computers from (5) internet centers. The swabs revealed the growth of Staph. Aureus isolates (70). Ecoli (85), Candida albicans (70), Streptococcus viridian's (67), Bacillus cereus (55), Proteus spp. (52) and Pseudomonas aeruginosa (18). The isolates demonstrate that the computer keyboard considers as a reservoir for pathogenic microorganisms and can transmit diseases from one person to another, this study referred to the importance of cleaning hands or using disinfectants before using the computers.