Expression and Polymorphism of Long Non-Coding RNAs in Colorectal Cancer

Abstract

Aim of work: to conduct a clinical biochemical study that aids in investigation of some non-coding RNA expressions and polymorphisms (including long non-coding RNAs and miRNAs) namely, PVT-1 and miR-186 in an attempt to provide new noninvasive diagnostic biomarkers for colorectal cancer(CRC)of the Egyptian patients.

Patients and methods: Eighty CRC studies and thirty healthy controls were included. Laboratory and pathological investigations were assessed. Serum miR-186 and long non-coding PVT-1was measured as well as genotype for PVT-1rs13255292.

Results: The CRC patients had a mean age of 50.91±12.0. The mean serum miR-186 level in CRC patients (1.38±0.21) was significantly higher than in the control (0.93±0.1) (p=0.0001). The PVT level in CRC patients was (5.91±0.25) significantly higher than control (1.1±0.2) (p=0.001). The PVT-1 rs13255292 genotype CT was significantly higher compared to the control group. The T allele was highly significant with p-value 0.008 in CRC patient group as regards to the control.

Conclusion:miR-186, long non-coding PVT-1 as well as PVT-1the T allele may be considered genetic markers for CRC susceptibility.