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The present study, was conducted to investigate the effect of the crude Flavonoid, Alkaloid, and Terpenoid, compounds extract from the leaves of (Myrtus communis L.) against pathogenic bacteria isolated from hemodialysis fluid in some hospitals in Hillah City 2020 in Iraq. Antibacterial activity was achieved in vitro by using agar-well diffusion method against pathogenic bacteria isolated from hemodialysis fluid by preparing three concentrations for each crude compound (50, 100, and 200) mg/ml and compared with positive control represented by Azithromycin antibiotic and negative control represented by 10% dimethyl sulfoxide. the aimed of this study to investigate the antibacterial efficacy of the Secondary Metabolites Extracted from Myrtus communis L. leaves against some pathogenic bacteria isolated from Hemodialysis Fluid. The data collected from the study revealed that, the crude Flavonoid and Alkaloid compounds extract from the leaves of (Myrtus communis L.) showed significant reduction at P≤ 0.05 in the growth of pathogenic bacteria isolated from Hemodialysis at 20 mg/ml compared with negative control. Finally, it can be concluded that Flavonoids and Alkaloid of Myrtus communis L. is most effective in controlling pathogenic bacteria isolated from hemodialysis fluid. While, terpenoid compounds was the least effective in controlling growth of pathogenic bacteria isolated from hemodialysis fluid compared with flavonoid and alkaloid.