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In order to determine the optimal conditions for the extraction of flavonoids from the seeds of basil (Ocimumbasilicum L. ) and fenugreek(Trigonella sp.), a group of factors affecting the extraction process on the base of flavonoid yield were studied. the optimal values of studied factors were detected which includes extraction solvent concentration, extraction temperature, and extraction time, using three different extraction methods included Conventionalextraction, ultrasonic assistedextraction and Maceration-vortex extraction. The chemical constitutes for both herbs were screened by GC-Mass technology. results showed that using the Conventionalextraction method by magnetic stirrer with hot plate with 80% methanol, at 45 and 55 C̊ for extended periods about 75 and 60 min gave the highest yield of glycosidic flavonoidsin which it scored 169.6 and 252.4 mg/g for basil and fenugreek respectively. While using the ultrasonic extraction method by ultrasonic water bath with 60% and 40% methanol, at 45 C̊ for extended periods about 60 min gave the highest yield of aglyconesflavonoids in which it scored 289.8 and 398.2 mg/g for basil and fenugreek respectively. GC-MS screening showed the presence of 57 bioactive compounds in fenugreek and 38bioactive compounds in basil.