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Background: Total knee arthroplasty (TKA) is a viable treatment for symptomatic knee osteoarthritis that is resistant to conservative measures. A potentially promising alternative option for cartilage regeneration in those with degenerative end stage modifications that damage the articular cartilage involving multiple knee compartments is yet to be reported in the literature. Study Aim: The aim wasto assess the nurses' knowledge concerning Total Knee Arthroplasty (TKA), Al Hussein Teaching Hospital. Method:A quantitative descriptive design was used with a cross sectional sample. In total 50nurses inAl muthanna Hospitals self-administered a questionnaire entitled "Nurses' Knowledge Concerning Knee Arthroplasty". The questionnaire included six domains to assess nurses’ knowledge concerning total knee arthroplasty. Results:There were no statistically significant relationships found between nurses’ knowledge regarding care of patients undergoing TKA and select nurse demographics. Conclusion:the researcher found decrease innurses’ knowledgeregarding the patient care needs of those undergoing TKA and non-significant association between demographic data and nurses’ knowledge. Recommendations: Periodic availability of unique pre-service and in-service training programs about knee arthroplasty for updating nurses' knowledge regarding TKA.