Immunological and molecular detection of Interleukin-1β(IL-1β) in Iraqi Women with Polycystic Ovarian Syndrome

Abstract

Background: Interleukin-1 considered as a cytokine type multifunction and it is a feature that causes inflammation. Aim and Objective: to detect and study the immunological and molecular affect of Interleukin-1β(IL-1β) in Iraqi Women with Polycystic Ovarian Syndrome. Materials and Methods: 250 Iraqi women, their ages were from 20 to 50 years, they were divided into two groups: study group (n=125, Polycystic Ovary Syndrome PCOS) while the other group (n=125 control group mainly normal women), blood samples weretaken in the second menstruate cycle day, the concentration of IL-1β wascalculated for each group, and after this determine the IL-1β SNP (IL-1β rs16944 A/G) by using real time PCR. Results: PCSO patients show a noticeable difference compared with other group. The mean level of IL-1β was 69.66±93.39 (Pg /ml), 3.52E2±139.15 (Pg /ml) in PCOS and healthy control respectively. The levels were ranging between 0 and 598 Pg/ml. The GG genotype (mutant gene) was detected in 94/125 (75.2%) of PCOS, and the AG genotype was detected in 31/125 (24.8%) of PCOS. Conclusions: the results showed high significant association of G allele in IL-1β rs16944 gene SNP (A/G) with PCOS, (P<0.0001), this increasing may related to ovulation lack, while the polymorphism in IL-1β-511A/G, can be a predisposeaspect for the PCOS weakness.