Histopathological and Immunohistochemical Study in Liver of Mice Muse Musculus Treated with Β-Carotene

Abstract

β-Carotene is one of the natural bioactive products that has received attention as additives to some cancer-suppressing drugs, so the current study aims to detect the relationship of this substance in stimulating the pathways of apoptosis. The present study evaluated the role of β-Carotene in vivo mice, it included detection of histopathological changes in the liver of mice Mus musculus treated with β-Carotene, and immunological detection of apoptosis proteins (caspase 8, caspase 9 and p53) in mentioned organ. There are variety of reversible and irreversible histopathological changes were observed in liver of mice treated with 300 μg/ml of β-Carotene while the samples that treated with 150 μg/ml did not suffered irreversible changes. The immunohistochemistry assay of apoptotic proteins (caspase 8, caspase 9 and P53) showed staining caspase8 protein in Large areas of liver tissue, of mice treated with high concentrations of β-Carotene, with brown of DAB stain, while the caspase9 and p53 proteins were stained in small separate area of tissue. In conclusion, the β-Carotene has a toxic effect with high concentrations on liver tissue and have no toxic effect in low concentration. The injury of tissue as a result the potential of the β-Carotene to induce apoptosis.