Association between IL-6 Gene Polymorphism in Position -174 G/C and Bladder Cancer Risk in Iraqi Population

Abstract

Our study included two hundred samples of which 120 patients of bladder cancer in Iraqi population, their ages ranged from 30 to 74 year (ages mean 44.83 ± 1.2) and 80 controls (healthy), their ages ranged from 28 to 67 year (ages mean 33.25±1.7). We confined the frequency of IL-6 Gene polymorphism in position -174 G/C by Tetra ARMS -PCR technique (Tetra-amplification refractory mutation system-polymerase chain reaction technique). Also, we determined the association of IL-6 Gene polymorphism in position-174 G/C with bladder cancer of Iraqi community. Statistical data showed significant difference in genotype frequency of IL-6 Gene polymorphism in positoin-174 G/C with patients and control (healthy). The genotype of CC (homozygotes) show high frequency in bladder cancer patients was ratio 50%, comparison with control was 22.50%, and showed association with etiological fraction risk of bladder cancer. The genotypes of GC (Heterozygotes) and CC (Homozygotes) show low frequency in bladder cancer ratio was 24.17% and 25.83% respectively, while in control show high frequency was 48.75% and 28.75% respectively, also GC and CC genotypes showed relationship with preventive fraction (PF) risk of bladder cancer patients. The G allele show high in frequency of bladder cancer patients comparison with control ratio was 62% and 46.88% respectively, and present with etiological fraction risk (EF) of bladder cancer patients in Iraqi population, while C allele show low frequency in bladder cancer patients comparison with control was 37.92% and 53.13% respectively, and present related with preventive fraction (PF) of bladder cancer patients. Our findings demonstrate that the IL-6 gene in position -174 G/C may represent of bladder cancer risk development of patient’s in Iraqi population.