Enhancement of Survival and Uranium Bioleaching and Bio-Sorption Properties of Bacteria by Immobilization Techniques in Egypt

Abstract

The Egyptian bacterial strain E. coli was isolated from Uranium Ore and used for bioremediation studies. Capsulated in calcium alginate beads, A Burette-flow- packed bed made of these beads was used for bio-sorption of U by rate of 968 ppm U m-3 water per day with a sorption efficiency of 93.2% was achieved with studying optimum condition for bacterial growth and bio-sorption process and bioleaching processes. Various isotherm models of adsorption such as Langmuir, Freundlich, were studied. The results predicted adsorption in a multi- layered via physio-sorption. In this study 6 bacterial isolate, isolated from Uranium Ore, from Aborshid Egypt, were characterized for their response to 15 antibiotics and 10 heavy metals beside uranium. Multiple resistant isolates were observed, the results revealed a varying response of the Ore bacteria to the tested U & heavy metals. All isolates demonstrated numerous metal protections towards 10 heavy metals, with MIC ranging from 50 to 1000 ppm. The strongest of the strains in both groups were resistant to Pb, Ni, Cu and Zn. Highly metal-resistant bacteria could be used with potential application for treatment of wastewater using immobilized bacterial cells, the most potent isolate was identified. The Egyptian strains belong to E. coli, based on 16S rRNA gene sequencing the nucleotide sequences reported here were deposited to the NCBI Nucleotide Sequence Database under accession numbers (MF496270) by the name of MostafaGomaaFadl this result confirmed by scan (EDX) FT-IR ICP device. Potent metal bio-sorbents among microorganisms, at low pH esteems, cell divider ligands are protonated and contend essentially with metals for official. With expanding pH, more ligands, such as amino and carboxyl groups, could be exposed, leading to attraction between these negative charges and the metals, and consequently increment bio-sorption onto the cell surface. Starting with Isolation and identification of heavy metal-resistant bacteria from rock Ore. Studying Factors Affecting Uranium Bio-sorption, Optimization of bacterial growth conditions and optimum for metal uptake by free and immobilized bacterial cells and Desorption ratio of uranium ions adsorbed by E. coli /alginate, All this evidence suggest that functions groups Represented in our study are responsible for metal uptake in our bacterial biomass beside change in peaks position which assigned for its groups confirm bio-sorption of metal ions from waste due to ions charge interaction comparing with immobilized we found increase in no of binding sites indicate that immobilized bacterial have high efficiency for metal up take which also change in peaks position which assigned for its groups confirm bio-sorption of metal ions from waste due to ions charge interaction.