The Study of Different Doses of Saccharin on Biochemical Parameters in Male Wister Rats

Abstract

Background: Saccharin is one of the world's most frequently used chemical sweeteners. Objectives:This study aims to investigate the effect ofSaccharin on certain parameters of biochemistry. Methodology: Sixty mature male Wistar rats were divided randomly into four groups, 15 for each group. The first control group dosed by distal water orally and the three treatment experimental groups (T1, T2, and T3) treated by saccharin solution orally at doses of (250, 500, and 750) mg/kg per day, respectively, for each rat, in one ml. All these classes were viewed on a regular basis for 90 days. After the end of experiment , blood sample was collected from for estimation body weight and blood glucose concentration. also the oxidative stress investigation was done by measuring catalase, peroxidase and glutathione. Results : The results of this study showed that there were significant increase (P< 0.05) in glucoseconcentration of (T1, T2 and T3) groups when compared with control group. When compared between treated groups T3 was more effected as compared with (T1 and T2).On the other hand, The results showed there were significant decrease (P<0.05) in glutathione level and catalase of (T1, T2 and T3) groups when compared with control group. On the other hand (T3) group treated was more effected as compared with (T1 and T2) groups. The current study showed there was significant increase (P< 0.05) in peroxidase concentration of (T1, T2 and T3) groups when compared with control group However, (T3) group was more effected as compared with (T1 and T2) groups. Conclusions :The statistical analysis for the results of body weight in this study showed that there was a significant decrease (p<0.05) in body weight of all treated groups compared with control group. (T3) group was more effected as compared with (T1 and T2) groups. From this study we concluded that Saccharin has a harmful effect on biochemical parameters.