Associations of KCNQ1 (rs2237892) Polymorphisms with the Risk of Type 2 Diabetes Mellitus in a Sample of Iraqi Patients

Abstract

This study was aimed to detect KCNQ1 gene polymorphism in Iraqi type 2 diabetes mellitus patients (T2DM) to found the correlation between the SNP (rs2237892) polymorphism in KCNQ1 gene and lipid metabolism and impact on the incidence of type 2 diabetes mellitus (T2DM). The result show high Significant difference was observed in FBS level (P<0.001) in patient group (172.9 ± 79.73) and in control (81.16 ± 7.18),). Cholesterol, LDL-Cholesterol and VLDL-Cholesterol mean level value in diabetic patients was significantly higher than those of control group (p<0.0001), serum HDL-Cholesterol mean value was significantly difference (P<0.05) in serum mean level of HDL between T2DM patients and healthy controls. Real time PCR (HRM) RT-PCR were used to detect SNP (rs2237892) in KCNQ1 gene (T>C) by using specific primers, as a related with SNP (rs2237892) T>C in KCNQ1 gene, genotypes and alleles frequencies, odds ratios, 95% confidence intervals and P values for the KCNQ1 gene In this study, statistical analyses of genotypic frequencies for the KCNQ1 (rs2237892) revealed significant (P<0.001) difference between T2DM patients and controls in the examined population. . The observed C/C, C/T, and T/T genotype frequencies were 96%, 4%, and 0%, respectively table (4-5). The C (wild-type) and T (variant) allele frequencies were 98% and 2% , respectively in patient group while the observed C/C, C/T, and T/T genotype frequencies were 72%, 28%, and 0, respectively and the C (wild-type) , T (variant) allele frequencies were 86% and 14%, respectively in control group (P <0.01). The KCNQ1 CC genotype were significantly (P <0.001) more frequent in the patient group. Conclusion: There was a relationship between polymorphism of KCNQ1 gene SNP (rs2237892) and the incidence of T2DM in sample of Iraqi patients.