Determination of Bioactive Compounds and Antibacterial of Spirulina sp. Extract

Abstract

Objective of this study was to determine of bioactive compounds and antibacterial activity of Spirulina sp extract. Pathogenic bacteria, including Streptococcus mutans, Staphyloylococcus aureus, Morganella morganii, Salmonella Sp.,Pseudomonas aerogenosa and Escherichia coli, as the active chemical compounds were extracted from this algae using solvents (cold water and methanol alcohol) with qualitative detection of secondary metabolites of the aqueous extract of this algae, which indicated the presence of phytochemicals analysis such as Alkaloids, Phenols, Flavonoids, Glycosides, Terpenoids, Taninns, Saponins and Carotenoids . The chemical compounds were determined using the gas chromatography mass spectroscopy (Gc-mass) technique of the methanolic extract of spirulina These compounds were represented Muramic acid, Isopropyl Alcohol, Diglycerol, Neophytadiene, n-Hexadecanoic acid, Heptadecanoic acid, Phytol, 9,12-Octadecadienoic acid (Z,Z)-, 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-, Linoleic acid ethyl ester and Floxuridine . The aqueous extract of this algae did not show any bioactivity against all bacterial species under study. It was noticed that the methanolic extract was more efficient in its effect on the bacterial species, as the rates of inhibition zones for this extract were (23.67, 21.33, 20.67, 18.00, 19.56 and 17.00) mm for each of S. mutans, Staph. aureus, M. morganii, Salmonella Sp. , P. aerogenosa and E. coli, respectively.