Main Article Content
Background and objective: The present work aims to the done modified method of herbal chitosan nanoparticle to improve the garlic nanoparticles extraction by decreasing the side effects of the aqueous extract. Methods:Garlic loaded chitosan nanoparticles (GCNPs) were prepared by combination of emulsification-solvent evaporationwith sonication method and slightly modifications into three forms, garlic (GNPs)), chitosan (CNPs), and garlic-chitosan (GCNPs). Three types of NPs were characterized by FESEM for particle size (PS) and zeta potential (ZP), FTIR, drug loading (DL%), encapsulation efficiency (EE%), and in vitro extract release. Results:GCNPS particle size was PS, 151.9±12.7 nm, PDI 0.152, and have ZP, -23.2.5. The higher DL% content of EV was detected as 7.32±1.1% and EE% was 87± 2.6% in formulation B. The results suggested that the GNPs dissolution was 80% within 4h but the release profiles of GCNPs form within 4h were 34, while GNPs and CNPs were 23, 26%, respectively. Conclusion:GCNPs were synthesized and characterized in specific properties for next labs animal experiments.