Molecular study to the fungus Neosetophomasamarorum was isolated from Al Chabaish marsh, South of Iraq دراسة جزيئية للفطر Neosetophomasamarorum المعزول من هور الجبايش جنوب العراق

Abstract

Neosetophomasamarorumis one of the phoma fungi put up with Ascomycota isolated from sea phoma. In this study,N. samarorumwas isolated fromAl Chabaish marsh, south of Iraq.This research aimed to the fungus Neosetophomasamarorum's molecular characterization using ITS gene and phylogenetic structuring tree.From35 samples of water collected from a different marsh area, eight samples were positive to Neosetophomasamarorum when cultured on PDA medium at 28C°, and 27samples were negative fungus. Genetic diagnosisresults for the fungus used specific primers for the ITS gene; thisdesign, especially for this project,showed that 8 (22.8%) were positive. The fungus was diagnosis depending onthe cultural feature and microscopic examination, and then PCR technology was used to ensurethe diagnosis of this fungus. Primers (ITS) and phylogenetic structuring treeanalysis were done by sequences and confirmation ofmicroorganism’s homogenous data using the database (NCBI) after amplifying Fungi’s ribosomal RNA. The resultshowed the genetic affinity percentage of N. samarorum between Germany and Canada is 99%, then among Poland, Canada and Germany are 99%, while between Netherland and Netherland is 96%, between Iran and China is 98%, and between Iran and China and Netherland are 96%.