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This study aims to formulate a vitamin C concentrate product with quercetin from mango (Mangifera indica) as a health supplement that can be used as an anticancer, antidiabetic, antihypertensive, and immunomodulator. Samples consist of 3 formula variations based on the ratio between mango and CO2-free water (S1 = 1:0,5; S2 = 2:1; S3 = 3:2). The two main ingredients are mixed and stirred using a blender-type mixing machine for 15 minutes and then put in a freezer for 12 hours with a temperature of -20°C to -23°C. After that, it is dried using a freeze dryer in 0.100 mbar for 60 hours. The product becomes 100% dry which is then encapsulated. Vitamin C analysis was done using the Titration Iodometric Method to determine the amount of Vitamin C (mg/100g), while the quercetin was determined with HPLC. The formulation of a vitamin C concentrate supplement product with quercetin from mangoes is stable and has been based on tests using iodometric-titration techniques against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and using high-performance liquid chromatography (HPLC). The obtained Vitamin C dan quercetin amount in each of the samples, S1 was 110.00 ±0.05 mg/100g and 41.93 μg/g, S2 was 130.00 ±0.20 mg/100g and 59.00 ±0.50 μg/g, and S3 was 165.00±0.01 mg/100g and 79.03± 0.08μg/g. S3 has the highest Vitamin C and quercetin content, which is potential to be produced commercially in the form of supplements.