Main Article Content
The constant influence of inadequate chemical, biological and other factors on the animal organism causes a stress reduction in resistance and an immunosuppressive state in animals. In the body, it is characterized by a deficit in the energy supply of the function of the genetic apparatus and enzymes, a toxic blockade of the specific activity of enzymes, the prevalence of catabolic processes and the stay of the body in a state between norm and pathology.
The aim of the research was to study the effectiveness of the use of α- and γ-interferons alone and in combination with dimethyldipyrazolyl selenide for the correction of the oxidative-antioxidant status in cows under environmental conditions.
Materials and methods. The studies were carried out on three groups of cows taken into the experiment 3 weeks before calving, of which one group (n = 12) served as a control without the use of drugs, the animals of the other two groups were prescribed pharmacologic agents: one of them (n = 12) - only α - and γ-bovine recombinant interferons and other (n = 14) - α- and γ-interferons in combination with dimethyldipyrazolyl selenide. Blood samples were taken from 5 cows from each group before the administration of drugs and 4 days after the last injection to determine the content of malondialdehyde, indicators of endogenous intoxication and antioxidant protection.
Results and Discussions. Cows with the administration of α- and γ-interferons alone and in combination with dimethyldipyrazolyl selenide, compared with animals in the control group, contained less malondialdehyde, respectively, by 12.7% and 21.8%, MSM at a wavelength of 238 nm - by 4 , 1% and 6.3%, MSM at a wavelength of 254 nm - by 12.5% (p <0.05) and 15.6% (p <0.05), SMP - by 13.5% and 8 ,one%. 21.6% (p <0.05). Of the indicators of the antioxidant defense system, they had higher values of glutathione peroxidase (GPO) activity by 4.8% and 14.0%, catalase - by 5.2% and 9.9%, vitamin A - by 8.8% and 10.4%, vitamin E - by 12.5% and 9.5%, vitamin C - by 14.6% and 17.0%. Generic pathology was recorded less often 3.0 and 3.5 times, postpartum - 2.0 and 4.7 times. The period from calving to insemination was shorter by 11.9 and 23.0 days, fertility was higher by 8.3% and 9.6%, the insemination index was lower by 15.4% and 26.9%.
Conclusion. Thus, the use of recombinant bovine α- and γ-interferons in cows in the last month of pregnancy alone and in combination with dimethyldipyrazolyl selenide under conditions of environmental disadvantage contributed to a decrease in the accumulation of a toxic product of lipid peroxidation in the body of animals - malondialdehyde, a decrease in the manifestation of endogenous intoxication, stabilization of the antioxidant system. protection, which had a positive effect on the state of the reproductive system.