Evaluation of the Mixture of phoenix Dactylifera Seeds extract and Chalcone Derivativeas Anti-Angiogenic Agents in Ex-Vivo Rat Aorta Ring Model

Abstract

Angiogenesis is very important in the progression of various pathological disorders, most importantly tumor growth and metastasis. This research aims to investigate the efficacy of the synthetic chalcone derivative)2-Hydroxychalcone)and its mixture with ethanolic extract of Phoenix dactylifera seeds as anti-angiogenic agents. The synthetic chalcone derivative was synthesized according to Claisen-Schmidt condensation method using SOCl2/ETOH as a catalyst with a good yield. The structure of the synthesizedchalcone derivative has been characterized by TLC, melting point, UV, and IR Spectroscopy , and elemental microanalysis (CHNO). The powdered seeds of P. dactylifera were extracted by soxhlet extraction with Ethanol. The synthetic chalcone derivative alone at concentration (17.15μg/ml) and its mixture with ethanolicextract of P. dactylifera seeds were evaluated for its anti-angiogenic activity using the ex-vivo rat aorta ring model at concentrations 17.15μg/ml and 30.9μg/ml respectively. At the previously mentioned concentrations, The synthetic chalcone derivativewas able to inhibit growth of blood vessels significantly (56.23%) in rat aorta ring assay in comparison with the negative control (P<0.05), while the mixture of the synthetic chalcone derivative and ethanolic extract of P. dactylifera seeds showed higher percentage of inhibition (76.63%)than that of the synthetic chalcone derivative alone in comparison with the negative control (P<0.05).Thisproduced effect could be due to the synergism between the synthetic chalcone derivative and the phytochemical constituents present in ethanolic extract in inhibiting different angiogenesis signaling pathways.