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This study analysed the molecular docking of the SilverOxide Nanoparticles (Ag2O-NPs) synthesized from the novelmedicinal plant Lagerstroemia indica.The formation of Ag2O-NPs was observed at a peak of 446 nm by the Ultraviolet-Visible (UV-Vis) spectrophotometerand the particle size was found to be9.98 nmby the X-Ray Diffraction (XRD) spectroscopy. Spherical-shaped NPs were detected from the Scanning Electron Microscope (SEM) images and the compound purity was observed from Energy Dispersive Spectroscopy (EDS) analysis. The weight percentage of Ag and O were found to be73.64 and 26.36,respectively. The atomic percentage of Ag and O were 29.29 and 70.71,respectively. The molecular docking of Caspase-3 was performed to determine the binding affinities of the Ag2O-NPs which helps in analysingthe apoptoticpotentialon human cancer cell lines.