Prolonged Effect of Tea and Coffee Consumption on Danio Rerio Behaviour and Bone Density

Abstract

World consumption of coffee among individuals has steadily increased by 1.3% per year, next to tea with an annual growth rate of 2%. Both appear to have some effect on the women’s bone density, which could lead to osteoporosis. This research evaluates the tea and coffee cytotoxicity toward bone density using zebrafish. Adult zebrafish were treated with 500mg/ml, 200mg/ml, 100mg/ml, 50mg/ml and 25mg/ml concentrations to determine the tea and coffee efficacy by visualizing bone anatomy and physiological changes. Zebrafish embryos were treated with 75mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml, 6.25mg/ml and 3.125mg/ml concentrations for cytotoxicity effects including the abnormal heartbeat,  high mortality rate,  pericardial oedema, somite and tail deformation. Histological findings do not interpret any significant changes in bone after prolonged consumption of tea and coffee. Behaviour pattern, yet reveals the zebrafish’s anxious response. Overall, the prolonged effect of tea and coffee have shown ambiguous results that may contribute to early osteoporosis development.