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Inthepresentcommunicationasynthesizedligand[2,3-dimethylanilineazothiobarbutaricacid](DMAT)wasusedtopreparethreenewcomplexesof(Co(II)Ni(II)andCu(II))Complexes.Theligandanditscomplexeswerecharacterizedbyelementalanalysis(CHN),UV-Vis,FTIRandHNMRspectroscopy, magnetic susceptibility measurement and molar conductance. The optimal condition (PHand concentration) and mole ratio for the preparing complexes were also studies. All data was showedthat the ligand acts as N,O- bidentate and all complexes have the composition of (1:2) (M:L) chelatestype and octahedral geometry. All prepared complexes are non-electrolytes. The pathogentic evaluationfortheligand (DMAT)and itscomplexes wereexperienced invitro againsttwo typeof bacteria.