Genotyping of Cryptosporidium Spp. in Domestic Dogs and Humans in Karbala Province Iraq.

Abstract

In this study, Cryptosporidium sp. was diagnosed in Domestic dogs and humans in Karbala province in Iraq by molecular detection by using a nested polymerase chain reaction (nPCR) by outer primer 18s (9005bp) and inner primer 18s (830bp) were used. our study was beginning of December (2019) to September (2020). A total of 100 fecal samples were collected from adult and young and from both sexes of dogs and 100 stool samples were collected from adult and young and from both sexes humans. The sequence study for 10 samples from the positive samples recorded at the National Center for Biotechnology Information (NCBI) was found 7 species C. parvum under accession number (MT329015.1 - MT329013.1) and 3 samples C. canis under accession number (MT329014.1, MT329016.1, and MT329018.1). The result of the sequence analysis In humans for 10 samples from the positive samples recorded at the (NCBI) was indicate 10 isolate Cryptosporidium parvum. under the accession numbers (MW186198 - MW186207) ).