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This experiment was conducted in the poultry farm of the Animal Production Department at the College of Agriculture - Al-Qasim Green University for 8 weeks from 1/10/2020 - 1/12/2020, preceded by two weeks to train roosters to give semen, and the experiment aimed to study the effect of Ross broiler breeder were dosed with different levels of nano-selenium and organic selenium particles in reproductive and biochemical characteristics. The stoke of the breeder (Ross 308) was used at the age of (64) weeks, and the roosters were divided randomly into 7 treatments with 5 replicates for each treatment and each one rooster included, and the treatments were as follows (0, 1 mg/kg organic selenium feed, 1.5 mg/kg organic selenium feed, 2 mg/kg organic selenium feed, 0.5 mg/kg nano selenium feed, 0.75 mg/kg nano selenium feed., 1 mg/kg nano-selenium feed) for the treatments (T1, T2, T3, T4, T5, T6, T7), respectively, and obtained the following results: Significantly excelled (P<0.05) for treatment T5 in sperm concentration, group, and individual motility. The concentration of FSH, LH, and Glutathione Peroxidase compared to the rest of the treatments. Significant (P<0.05) for treatment T7 in the proportion of live sperm and concentration of testosterone hormone, and its significant improvement in the percentage of dead sperm compared to the rest of the treatments. Significantly excelled (P<0.05) for all treatments of nano-selenium and organic selenium in the ratio of sperm resistance to salt compared with treatment Control, significant superiority (P<0.05) for treatment T2 in the absolute weight of the testis, significant superiority (P<0.05) for treatment T1 in estrogen concentration compared with the rest of the treatments.