Isolation and Identification of Cellulytic Ruminant Pseudomonas Aeruginosa by Classical and Molecular Methods in Basrah Province

Abstract

Cellulose is the most abundant sugar on the surface of the earth and is the main building material in plants that is the primary link in what is known as the food chain. Cellulose is also found in a variety of other organisms, such as algae, fungi, and bacteria. Cellulose is a major component of the ruminant diets and can be broken down into metabolites that can be absorbed by the host for use as an energy source. This study was conducted to isolate and diagnose some cellulose-analyzed bacterial species present inside the ruminant rumen, where the number of samples was 30 samples distributed between (3) samples of camels (10) samples from sheep (4) from buffaloes (13) samples from cows, which were collected from the Basra and Najaf slaughterhouses, The isolates were diagnosed by biochemical and phenotypic tests, and then their diagnosis was confirmed by molecular methods by means of the 16SrRNA diagnostic gene by polymerase chain reaction (PCR). The molecular and phenotypic results showed that the number of isolates was 12 isolates distributed among the bacterial species where the number of Pseudomonas aeruginosa was three isolates by 25%.