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Introduction & Objective:Increased clinical dentin hypersensitivity is seen among 30-40-year-olds. This complication is directly related to the size of the dentinal tubules. Therefore, considering the importance of dental sensitivity control and finding newer and more mundane ways to control it. The purpose of this study was to investigate the effect of bioglass 45 s5 and calcium oxalate (gluma) for covering the dentinal tubules in dental sensitivity. Methods: This experimental and laboratory study was conducted at the Army Dentistry School in 2019, after obtaining permission from the Ethics Committee of the Army Medical University. For this study, 45 premolar and healthy molars were collected from the surgery department of the Army Dentistry School. The teeth lacked any restorations, caries, or cracks. Samples were randomly assigned into three equal groups using the random numbers table. A group was treated with bioglass, a group treated with gluma (calcium oxalate), and a group was as the control group. After collecting data from the samples using a questionnaire, the information was encrypted and entered into the computer. Then, using SPSS software, one-way analysis of variance was used to analyze the data. Normality analysis of the mean distribution was performed using the Kolmogorov-Smirnov test. In all tests, the significance level was considered to be 0.05. Results: Both gluma and bioglass materials wereeffective and significantly fillupDentin tubules more than control group thus can effectively reduce dental sensitivity. On the other hand, the results showed that Bioglass is significantly more effective than gluma. It causes dentinal tubules to fill up. So, in SEM images, Bioglass in few samples closed all dentin tubules; therefore, it seems to be more effective in reducing dental sensitivity. Conclusion: According to our results, both bivalve cells and gluma had a significant effect in dentin tubules compared to the control group. In addition, Bioglez is a more effective anti-allergic agent than gluma.