CAT , SOD and MDA Variations in the Spirulina platensisin the Presence of MPs

Abstract

the variation of Catalase (CAT), Superoxide dismutase (SOD), and Malondialdehyde (MDA) in Spirulina platensis microalgae due to the effect of four types of microplastics (Poly Ethylene, Poly Styrene and Poly Styrene Blue). CAT content values were found highest in the case of PVC (5.582 umol/L at 250 mg/L) and the lowest in the case of PE (3.92 umol/L at 250 mg/L) with a sequence of (PVC>PSB>PS>PE).On the other hand, SOD content values were found highest in the case of PVC (19.371 umol/L at 250 mg/L) and the lowest in the case of PE (17.85 umol/L at 250 mg/L) and the sequence of SOD content was found as follow (PVC>PSB>PS>PE).A deferent trend was obtained in the case of MDA, as the activity of MDA increased significantly continually until the last day of investigation with a small decrease at the last 5 days for the single treatment of (PE, PS, PSB, and PVC) as compared to the control treatment for all the range of concentration (12.5, 25, 50, 75, 100,125, and 250 mg/L) at a significance of (p<0.05). On the other hand, MDA content values were found highest in the case of PVC (18.64 umol/L at 250 mg/L) and the lowest in the case of PE (13.579 umol/L at 250 mg/L) and the sequence of MDA content was found as follow (PVC>PSB>PS>PE).The result of CAT content, SOD content, and MDA content recommended that the MPs presence can promote the microalgae cell to produce extreme ROS as it's confirmed by the increase of MDA content.