Detection of Blaoxa-23andblaoxa-51 Genes in MDR Acinetobacterbaumanniiisolated from Different Clinical Sources in Baquba Teaching Hospital in Diyala City, Iraq

Abstract

Acinetobacterbaumannii is a nosocomial pathogenic bacteria that is increasingly important also mainly affects ill people. In the present research, blaOXA-23 and blaOXA-51 genes have been observed in A. baumannii clinical MDR isolates. Methodology: From 22 A. baumannii isolates, separate clinical specimens were collected from (burns, wounds , blood, urine and fluids) at Baqubah Teaching Hospital. The isolates were biochemically identified and confirmed it by the compact device VITEK 2. The resistance of isolates against antibiotics was tested using the procedure of disc diffusion for all of the following antibiotics ( Amikacin ,Ampicillin sulbactam ,Ceftazidme, Ceftraxon , Ciproflaxacin , Colistinsulphate , Doxycylin ,Impenem, Levoflaxacin , Meropenem, Polymyxin B,TrimetheprimSulfomethazol). In order to identify blaOXA-23 and blaOXA-51 genes, conventional polymerase chain reaction(PCR) was accomplished. Results: This study found that of 22 isolates 11(50%) isolated from burns, 5(22.7%) blood, 3( 13.6%)wounds , 2(9%) urine and 1(4.5%) fluids. The current study showed that there was high resistance (100%) to antibiotics (Amikacin, Ciproflaxacin andLevoflaxacin) while the resistance of isolates toward antibiotics (Ampicillin sulbactam, Ceftraxon, Doxycylin, Empenem, Meropenem, Trimetheprimsulfomethazol) were (95%, 81%, 90%, 40% 86%, 90% and 77%), respectively. Whereas resistance to polymyxin B and colistin showed 0%. BlaOXA-51 was detected in 22 (100 %), while blaOXA-23 was detected in 18 isolates (81 %). Conclusion: The study showed a high prevalence of blaOXA-23 and blaOXA-51 genes among A. baumannii isolates which highlighted the importance of molecular study in order to find the appropriate management and to avoid their spreading during the hospital location